The purpose of this document is to ensure all thyroid gland specimens are described and dissected properly so that a diagnosis can be issued. Please refer to local standard operating procedures (SOP) for complete details.
(NOTE: You can view 3D models in larger format by clicking on the "fullscreen" icon at the bottom right.)
The learner is expected to apply some elements of the content learned in part 1 in the grossing of the simulation specimens.
Note: If the learner is unsure of the next steps during the simulation workshop, they should ask questions and/or seek help.
After reviewing the relevant readings, images, and SOPs and finishing the Introduction to Grossing Workshop, the learner will:
Confirm patient identification, specimen type, and alpha designation for the specimen
Read the provided clinical history
Orientate a thyroid, identifying the left lobe, isthmus, pyramidal lobe and right lobe.
Select and wear the appropriate PPE for the grossing of the simulation specimens.
Set up an organized grossing bench for the grossing of simulation specimens.
Follow and apply patient, specimen and tissue cassette identification procedures.
Describe the steps for grossing a thyroid AP04-9.01 Thyroid (labqms.com)
Gross a simulated thyroid using the thyroid SOP
Select the correct processing time for a thyroid
Demonstrate the safe setup, cleaning and discard of sharps (scalpel and feather blades)
Clean the grossing bench between specimens and apply steps to limit cross-contamination between specimens.
Determine when they are unsure of the next steps and ask questions/seek help.
The specimen is received fresh and is labeled PATIENT LAST NAME, FIRST NAME, designated Thyroid: Right Thyroid Lobe. The specimen consists of a right hemithyroidectomy (28.1 g; right lobe: 5.3 cm SI x 3.3 cm RL x 2.6 cm AP; isthmus: 2.0 cm SI x 0.9 cm RL x 0.6 cm AP). The external surface of the right lobe is inked blue, the external surface of the isthmus is inked orange, and the isthmic resection margin is inked black.
The external surface of the right lobe is smooth and grossly without identifiable skeletal muscle. Sectioning the lobe reveals a solid, well-defined, tan to hemorrhagic mass (3.7 cm SI x 3.1 cm RL x 2.6 cm AP) in the inferior aspect of the lobe, which abuts the external surface of the lobe and comes to within 0.9 cm of the isthmic resection margin.
The external surface of the isthmus is smooth and unremarkable. Sectioning reveals an unremarkable cut surface.
Photographs are taken.
Representative sections are submitted per the accompanying diagram as follows:
A1-A2. Isthmus in toto, perpendicular sections
A3-A11. Right lobe
A3. Superior pole, perpendicular sections
A4-A10. Lobe transverse sections superior to inferior
A5-A9. Mass
A11. Inferior pole, perpendicular section
AP04-9.01 Thyroid (labqms.com)
AP05-1.02 Peloris AttachA Tissue Size Chart
AP04-1.01 Dissection Guidelines AP04-1.01 Dissection Guidelines (labqms.com)
Please note: this is only a brief description of relevant anatomy that is most likely encountered at the time of dissection/grossing. For more complete details, please refer to complete and detailed anatomy references.
The thyroid gland is a butterfly-shaped endocrine organ located at the base of the neck. Comprising two lateral lobes connected by a thin isthmus, the thyroid is situated in front of the trachea, covering its anterolateral surfaces (including cricoid cartilage and the lower part of the thyroid cartilage). Structurally, the gland is composed of follicles, which are small sacs lined with thyroid follicular cells. The isthmus that connects the two lobes typically crosses the anterior surface of the 2nd and 3rd tracheal rings.
The thyroid gland is supplied by two major arteries: the superior thyroid artery (branch of the external carotid artery) and the inferior thyroid artery (branch of the thyrocervical trunk). Venous drainage includes the superior thyroid vein (drains area supplied by the superior thyroid artery) and middle and inferior thyroid veins that drain the rest of the thyroid gland. Superior and middle thyroid veins drain into the internal jugular vein.
Parathyroid glands are two pairs of small, ovoid, yellowish structures on the deep surface of the lateral thyroid lobes. Designated as superior and inferior parathyroid glands. However, their position can be quite variable.
Thyroidectomy is usually performed to remove nodules, either benign or malignant, benign follicular nodular disease (multinodular goitre), or for treatment of Grave's disease. Thyroidectomies can include the complete gland (total thyroidectomy) or only lateral lobes (hemithyroidectomy/lobectomy and isthmusectomy). Prior to surgery, nodules are typically evaluated by fine needle aspiration (FNA) for cytologic evaluation.
3D model provides anatomic representation of typical thyroid gland
3D model provides an anatomic representation of a typical thyroid gland with inked surfaces.
Ink code:
Right lobe - green
Left lobe - blue
Isthmus - orange
Total thyroidectomy specimens can be inked in different colours to differentiate left and right lobes and isthmus.
Separate the lobes from the isthmus. Serially section each lobe transversely from superior to inferior.
For total thyroidectomy (A), section isthmus in sagittal plane (vertical)
For hemithyroidectomy (C), section isthmus transversely
Illustrated images are adapted from:
Zakka FR et al. To Freeze or Not to Freeze? Recommendations for Intraoperative Examination and Gross Prosection of Thyroid Glands. Surg Pathol Clin. 2023 Mar;16(1):15-26. Epub 2022 Dec 10. PMID: 36739161.
3D model provides an anatomic representation of a typical thyroid gland with inked surfaces. The gland is sectioned. Note perpendicular sections for the superior and inferior poles.
Ink code:
Right lobe - green
Left lobe - blue
Isthmus - orange
Thyroid gland, section through papillary thyroid carcinoma (3D reconstruction)